Human Transcription factor Sp3, SP3 ELISA KIT
Product Name:Human Transcription factor Sp3, SP3 ELISA KIT
Packing:96T
Catalog No.:ELI-19951h
Gene Name:SP3
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:Transcription factor Sp3(SP3
Alternative Name:SP3,Transcription factor Sp3(SP3
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Transcription factor Sp3, SP3 ELISA KIT allows for the in vitro quantitative determination of Transcription factor Sp3(SP3 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Transcription factor Sp3, SP3 ELISA KIT has been pre-coated with an Transcription factor Sp3(SP3 antibody specific to Transcription factor Sp3(SP3 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Transcription factor Sp3(SP3 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Transcription factor Sp3(SP3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Transcription factor Sp3(SP3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!