Human Transitional endoplasmic reticulum ATPase, VCP ELISA KIT

Product Name:Human Transitional endoplasmic reticulum ATPase, VCP ELISA KIT
Packing:96T

Catalog No.:ELI-16208h

Gene Name:VCP

Detect Range:0.156-10 ng/mL

Sensitivity:0.078ng/mL

Target Protein Name:Transitional endoplasmic reticulum ATPase(VCP

Alternative Name:VCP,Transitional endoplasmic reticulum ATPase(VCP
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human Transitional endoplasmic reticulum ATPase, VCP ELISA KIT allows for the in vitro quantitative determination of Transitional endoplasmic reticulum ATPase(VCP concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human Transitional endoplasmic reticulum ATPase, VCP ELISA KIT has been pre-coated with an Transitional endoplasmic reticulum ATPase(VCP antibody specific to Transitional endoplasmic reticulum ATPase(VCP .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Transitional endoplasmic reticulum ATPase(VCP and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Transitional endoplasmic reticulum ATPase(VCP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Transitional endoplasmic reticulum ATPase(VCP in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!