Human TTN ELISA Kit
Product Name:Human TTN ELISA Kit
Packing:96T
Catalog No.:ELA-E1667h
Gene Name:TTN
Detect Range:1.56-100 pmol/mL
Sensitivity:0.78 pmol/mL
Target Protein Name:Human Titin
Alternative Name:Titin, Connectin, Rhabdomyosarcoma antigen MU-RMS-40.14, TTN
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human TTN ELISA Kit allows for the in vitro quantitative determination of Human Titin concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human TTN ELISA Kit has been pre-coated with an TTN antibody specific to Human Titin .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Titin and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Titin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm