Human USP39 ELISA KIT
Packing 96Tests
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
Gene Name USP39
Protein Name U4/U6.U5 tri- snRNP- associated protein 2
Alternative Name
Human USP39 ELISA KIT ,Human 65K ELISA KIT ,Human CGI-21 ELISA KIT ,Human HSPC332 ELISA KIT ,Human SAD1 ELISA KIT ,Human SNRNP65 ELISA KIT ,Human U4/U6.U5 tri-snRNP-associated protein 2 ELISA KIT ,Human SAD1 homolog ELISA KIT ,Human SnRNP assembly defective 1 homolog ELISA KIT ,Human U4/U6.U5 tri-snRNP-associated 65 kDa protein ELISA KIT ,Human inactive ubiquitin-specific peptidase 39 ELISA KIT ,Human small nuclear ribonucleoprotein 65kDa (U4/U6.U5) ELISA KIT ,Human ubiquitin specific protease 39 ELISA KIT ,Human ubiquitin specific peptidase 39 ELISA KIT
Human USP39 ELISA KIT Range 0.156-10ng/mL
Intended use
Human USP39 ELISA KIT allows for the in vitro quantitative determination of USP39 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
Reagent | Quantity |
Assay plate | 1 |
Standard | 2 |
Sample Diluent | 1 × 20mL |
Assay Diluent A | 1 × 10mL |
Assay Diluent B | 1 × 10mL |
Detection Reagent A | 1 × 120μL |
Detection Reagent B | 1 × 120μL |
Wash Buffer(25 x concentrate) | 1 × 30mL |
Substrate | 1 × 10mL |
Stop Solution | 1 × 10mL |
Plate sealer | 5 |
Test principle
The microtiter plate provided in Human USP39 ELISA KIT has been pre-coated with an USP39 antibody specific to USP39. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for USP39 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain USP39, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of USP39 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Sample collection and storage
Serum - Use a serum separator tube (SST) and allow samples to clot for 30 minutes before centrifugation for 15 minutes at approximately 1000 × g. Remove serum and assay immediately or aliquot and store samples at -20