Human UBE2A ELISA Kit
Product Name:Human UBE2A ELISA Kit
Packing:96T
Catalog No.:ELA-E0991h
Gene Name:UBE2A
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:Human Ubiquitin-conjugating enzyme E2 A
Alternative Name:Ubiquitin-conjugating enzyme E2 A, RAD6 homolog A, HR6A, hHR6A, Ubiquitin carrier protein A, Ubiquitin-protein ligase A, UBE2A, RAD6A
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human UBE2A ELISA Kit allows for the in vitro quantitative determination of Human Ubiquitin-conjugating enzyme E2 A concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human UBE2A ELISA Kit has been pre-coated with an UBE2A antibody specific to Human Ubiquitin-conjugating enzyme E2 A .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Ubiquitin-conjugating enzyme E2 A and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Ubiquitin-conjugating enzyme E2 A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm