Human Ubiquitin carboxyl- terminal hydrolase 30, USP30 ELISA KIT

Product Name:Human Ubiquitin carboxyl- terminal hydrolase 30, USP30 ELISA KIT
Packing:96T

Catalog No.:ELI-40419h

Gene Name:USP30

Detect Range:78-5000 pg/mL

Sensitivity:32pg/mL

Target Protein Name:Ubiquitin carboxyl-terminal hydrolase 30(USP30

Alternative Name:USP30,Ubiquitin carboxyl-terminal hydrolase 30(USP30
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human Ubiquitin carboxyl- terminal hydrolase 30, USP30 ELISA KIT allows for the in vitro quantitative determination of Ubiquitin carboxyl-terminal hydrolase 30(USP30 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human Ubiquitin carboxyl- terminal hydrolase 30, USP30 ELISA KIT has been pre-coated with an Ubiquitin carboxyl-terminal hydrolase 30(USP30 antibody specific to Ubiquitin carboxyl-terminal hydrolase 30(USP30 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Ubiquitin carboxyl-terminal hydrolase 30(USP30 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Ubiquitin carboxyl-terminal hydrolase 30(USP30, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Ubiquitin carboxyl-terminal hydrolase 30(USP30 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!