Human UCP2 ELISA Kit

Product Name:Human UCP2 ELISA Kit
Packing:96T

Catalog No.:ELA-E2066h

Gene Name:UCP2

Detect Range:0.156-10 ng/mL

Sensitivity:0.07 ng/mL

Target Protein Name:Human Mitochondrial uncoupling protein 2

Alternative Name:Mitochondrial uncoupling protein 2, UCP 2, Solute carrier family 25 member 8, UCPH, UCP2, SLC25A8
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human UCP2 ELISA Kit allows for the in vitro quantitative determination of Human Mitochondrial uncoupling protein 2 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human UCP2 ELISA Kit has been pre-coated with an UCP2 antibody specific to Human Mitochondrial uncoupling protein 2 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Mitochondrial uncoupling protein 2 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Mitochondrial uncoupling protein 2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human Mitochondrial uncoupling protein 2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!