Human Vacuole membrane protein 1, VMP1 ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

VMP1,EPG3; TANGO5; TMEM49; ectopic P-granules autophagy protein 3 homolog; transmembrane protein 49; transport and golgi organization 5 homolog; vacuole membrane protein 1

Search name

Human VMP1 ELISA KIT ,Human EPG3 ELISA KIT ,Human TANGO5 ELISA KIT ,Human TMEM49 ELISA KIT ,Human ectopic P-granules autophagy protein 3 homolog ELISA KIT ,Human transmembrane protein 49 ELISA KIT ,Human transport and golgi organization 5 homolog ELISA KIT ,Human vacuole membrane protein 1 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Human Vacuole membrane protein 1, VMP1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Vacuole membrane protein 1, VMP1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Human Vacuole membrane protein 1, VMP1 and Avidin conjugated to Horseradish Peroxidase(HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Vacuole membrane protein 1, VMP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human Vacuole membrane protein 1, VMP1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Customized Product