Human Von Hippel- Lindau disease tumor suppressor, VHL ELISA KIT

Product Name:Human Von Hippel- Lindau disease tumor suppressor, VHL ELISA KIT
Packing:96T

Catalog No.:ELI-06082h

Gene Name:VHL

Detect Range:0.312-20 ng/mL

Sensitivity:0.039ng/mL

Target Protein Name:Von Hippel-Lindau disease tumor suppressor(VHL

Alternative Name:VHL,Von Hippel-Lindau disease tumor suppressor(VHL
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human Von Hippel- Lindau disease tumor suppressor, VHL ELISA KIT allows for the in vitro quantitative determination of Von Hippel-Lindau disease tumor suppressor(VHL concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human Von Hippel- Lindau disease tumor suppressor, VHL ELISA KIT has been pre-coated with an Von Hippel-Lindau disease tumor suppressor(VHL antibody specific to Von Hippel-Lindau disease tumor suppressor(VHL .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Von Hippel-Lindau disease tumor suppressor(VHL and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Von Hippel-Lindau disease tumor suppressor(VHL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Von Hippel-Lindau disease tumor suppressor(VHL in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!