Human Wilms tumor protein, WT1 ELISA KIT

Product Name:Human Wilms tumor protein, WT1 ELISA KIT
Packing:96T

Catalog No.:ELI-40484h

Gene Name:WT1

Detect Range:0.156-10 ng/mL

Sensitivity:0.078ng/mL

Target Protein Name:Wilms tumor protein(WT1

Alternative Name:WT1,Wilms tumor protein(WT1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human Wilms tumor protein, WT1 ELISA KIT allows for the in vitro quantitative determination of Wilms tumor protein(WT1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human Wilms tumor protein, WT1 ELISA KIT has been pre-coated with an Wilms tumor protein(WT1 antibody specific to Wilms tumor protein(WT1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Wilms tumor protein(WT1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Wilms tumor protein(WT1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Wilms tumor protein(WT1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!