Human WNT1 Inducible Signaling Pathway Protein 2,WISP2 ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Human WNT1 Inducible Signaling Pathway Protein 2,WISP2 concentrations in cell culture supernates, serum, plasma, tissue homogenates and other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to WISP2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for WISP2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain WISP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of WISP2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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