Human WNT5A ELISA Kit

Product Name:Human WNT5A ELISA Kit
Packing:96T

Catalog No.:ELA-E1038h

Gene Name:WNT5A

Detect Range:0.156-10 ng/mL

Sensitivity:0.062 ng/mL

Target Protein Name:Human Protein Wnt-5a

Alternative Name:Protein Wnt-5a, WNT5A
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human WNT5A ELISA Kit allows for the in vitro quantitative determination of Human Protein Wnt-5a concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human WNT5A ELISA Kit has been pre-coated with an WNT5A antibody specific to Human Protein Wnt-5a .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Protein Wnt-5a and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Protein Wnt-5a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm