Human Zona pellucida sperm-binding protein 3, ZP3 ELISA Kit

FOR RESEARCH USE ONLY!

96 Tests

Alternative name

Human ZP3 ELISA KIT ,Human zona pellucida glycoprotein ZP3 ELISA KIT ,Human ZP3A ELISA KIT ,Human ZP3B ELISA KIT ,Human ZPC ELISA KIT ,Human Zp-3 ELISA KIT ,Human ZP3A/ZP3B ELISA KIT ,Human sperm receptor ELISA KIT ,Human zona pellucida glycoprotein 3A (sperm receptor)ELISA KIT ,Human zona pellucida glycoprotein 3B ELISA KIT ,Human zona pellucida protein C ELISA KIT ,Human zona pellucida glycoprotein 3 (sperm receptor)ELISA KIT

Intended use

Human ZP3 ELISA kit allows for the in vitro quantitative determination of Human ZP3 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in Human ZP3 ELISA kit has been pre-coated with an antibody specific to ZP3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for ZP3 and Avidin conjugated to Horseradish Peroxidase (HRP)is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain ZP3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of ZP3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Range 0.62-40ng/ml

Human ZP3 ELISA kit Manual download

Order Human ZP3 ELISA kit by email:[email protected]