Human ZP3 ELISA Kit
Product Name:Human ZP3 ELISA Kit
Packing:96T
Catalog No.:ELA-E11951h
Gene Name:ZP3
Detect Range:0.625-40 ng/mL
Sensitivity:0.156 ng/ml
Target Protein Name:Human Zona pellucida sperm-binding protein 3
Alternative Name:Zona pellucida sperm-binding protein 3, Sperm receptor, ZP3A/ZP3B, Zona pellucida glycoprotein 3, Zp-3, Zona pellucida protein C, ZP3, ZP3A, ZP3B, ZPC
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human ZP3 ELISA Kit allows for the in vitro quantitative determination of Human Zona pellucida sperm-binding protein 3 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human ZP3 ELISA Kit has been pre-coated with an ZP3 antibody specific to Human Zona pellucida sperm-binding protein 3 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Zona pellucida sperm-binding protein 3 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Zona pellucida sperm-binding protein 3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human Zona pellucida sperm-binding protein 3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!