ENTPD4 ELISA KIT|Human
Catalog EF009400
Packing 48T/96T
Reactivity Human
Detect Range 0.156-10ng/ml
ENTPD4 ELISA KIT Sensitivity< 0.094ng/ml
Application ENTPD4 ELISA KIT|Human is for detecting the concentration of Human ENTPD4 in serum, plasma, tissue homogenates and other biological fluids.
Storage condition 6 month at 4 Centigrade
Principle of ENTPD4 ELISA KIT|Human
The microtiter plate provided in ENTPD4 ELISA KIT|Human has been pre-coated with an Human ENTPD4 antibody specific to Human ENTPD4. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated Human ENTPD4antibody preparation specific for Human ENTPD4 then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human ENTPD4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm . The concentration of Human ENTPD4in the samples is then determined by comparing the O.D. of the samples to the standard curve.
*Please noted that ENTPD4 ELISA KIT|Human is only for RUO (Research Use Only), Not for the diagnostic Purpose!
Technical Principle & Methodology
The quality assurance and experimental performance of this biological reagent are rigorously optimized and standardized according to its specific technical pathway:
Double Antibody Sandwich Method
(双抗体夹心法). This ensures maximum reproducibility across clinical research and scientific workflows.
