Human Glutamyl aminopeptidase, ENPEP ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Glutamyl aminopeptidase,EAP,Aminopeptidase A,AP-A, ENPEP,glutamyl aminopeptidase A; glutamyl aminopeptidase; APA; CD249; gp160; AP-A; EAP; aminopeptidase A; differentiation antigen gp160, glutamyl aminopeptidase

Search name

Human Glutamyl aminopeptidase ELISA KIT ,Human EAP ELISA KIT ,Human Aminopeptidase A ELISA KIT ,Human AP-A ELISA KIT ,Human  ENPEP ELISA KIT ,Human glutamyl aminopeptidase A ELISA KIT ,Human glutamyl aminopeptidase ELISA KIT ,Human APA ELISA KIT ,Human CD249 ELISA KIT ,Human gp160 ELISA KIT ,Human AP-A ELISA KIT ,Human EAP ELISA KIT ,Human aminopeptidase A ELISA KIT ,Human differentiation antigen gp160 ELISA KIT ,Human glutamyl aminopeptidase ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Human glutamyl aminopeptidase,EAP concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to EAP. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for EAP and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain EAP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of EAP in the samples is then determined by comparing the O.D. of the samples to the standard curve.