Human Glycated Albumin, GA ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

GA, Albumin protein; HSA protein; Glycated Albumin protein, Glycated Albumin

Search name

Human GA ELISA KIT ,Human  Albumin protein ELISA KIT ,Human HSA protein ELISA KIT ,Human Glycated Albumin protein ELISA KIT ,Human Glycated Albumin ELISA KIT ,

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Human Glycated Albumin,GA concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to GA. Standards or samples are then added to the appropriate microtiter plate wells with Anchor a biotin-conjugated antibody preparation specific for GA and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain GA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of GA in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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