Human NLRP12 ELISA KIT
Product Name:Human NLRP12 ELISA KIT
Packing:96T
Catalog No.:ELI-16820h
Gene Name:NLRP12
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:Human NLRP12
Alternative Name:NLRP12,Human NLRP12
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human NLRP12 ELISA KIT allows for the in vitro quantitative determination of Human NLRP12 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human NLRP12 ELISA KIT has been pre-coated with an Human NLRP12 antibody specific to Human NLRP12 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human NLRP12 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human NLRP12, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Human NLRP12 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
Technical Principle & Methodology
The quality assurance and experimental performance of this biological reagent are rigorously optimized and standardized according to its specific technical pathway:
Double Antibody Sandwich Method
(双抗体夹心法). This ensures maximum reproducibility across clinical research and scientific workflows.