Human Polycystin-2,PKD2 ELISA KIT

Human Polycystin- 2, PKD2 ELISA KIT

96 Tests

Operating instructions

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

PKD2,Polycystin-2,APKD2; PC2; PKD4; Pc-2; TRPP2; R48321; autosomal dominant polycystic kidney disease type II protein; polycystwin; transient receptor potential cation channel subfamily P member 2; transient receptor potential cation channel subfamily P member 2; polycystic kidney disease 2, Polycystwin, Polycystic kidney disease 2 protein

Search name

Human PKD2 ELISA KIT ,Human Polycystin-2 ELISA KIT ,Human APKD2 ELISA KIT ,Human PC2 ELISA KIT ,Human PKD4 ELISA KIT ,Human Pc-2 ELISA KIT ,Human TRPP2 ELISA KIT ,Human R48321 ELISA KIT ,Human autosomal dominant polycystic kidney disease type II protein ELISA KIT ,Human polycystwin ELISA KIT ,Human transient receptor potential cation channel subfamily P member 2 ELISA KIT ,Human transient receptor potential cation channel subfamily P member 2 ELISA KIT ,Human polycystic kidney disease 2 ELISA KIT ,Human Polycystwin ELISA KIT ,Human Polycystic kidney disease 2 protein ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of human Polycystin-2 concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

Test principle

The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Polycystin-2, During the reaction, Polycystin-2 in the sample or standard competes with a fixed amount of biotin-labeled Polycystin-2 for sites on a pre-coated Monoclonal antibody specific to Polycystin-2. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Polycystin-2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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