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Extraction plasmids

Operation
1.Take the powder plasmid by centrifugation at 5000rpm/min for 1min.Add 20μl ddH2O in to the tube of plasmid.
2.Take 2ul plasmid in to 50ul competent cells, Place the mixture in ice water for 30minutes.Then Heat-shock for 90 seconds at 42℃.
3.Add it into liquid nutrient medium. then put on the Constant temperature shaker (180 rpm) for 45minutes.Spread the cell on the LB plate after centrifugal.
4.Place the plate in Incubator for 1-2 hours, then inverted and Incubate for 12-16 hours.
5.Take the Monoclonal colony on the LB medium,then put on the Constant temperature shaker for 12-16 hours at 37℃.
6.Extraction plasmids as your required.