Monkeypox Virus PCR Kit
Catalog No. 01vds-pcr 25T
Store at -20 ℃
-PRINCIPLE OF THE ASSAY
Monkeypox Virus PCR Kit is based on real-time PCR technology. Specific primers and probes are designed based on F3L gene areas of the Monkeypox virus. In addition, the kit also contains a housekeeping gene (RNase P) as an internal control (IC) for specimen sampling and nucleic acid extraction.
-Features
Feasible: compatible with multiple sample types；Reliable: 5 copies/reaction with internal control；Fulfilling: Central African clade & West African clade detectable;
-Content
| Nucleic acid extract |
1 x 1.8 mL |
50 / 100 μ L |
| Monkeypox virus nucleic acid fluorescent PCR detection mixture |
1 x 950 μ L |
35 μ L |
| Enzyme (Taq + ung) |
1 x 12 μ L |
0.4 μ L |
| H2O |
1 x 400 μ L |
|
| Internal control |
1 x 30 μ L |
1 μ L |
| Positive control (1) × 107 copies/mL) |
1 x 30 μ L |
1 μ L |
-Detect Range
1× 104～1× 108 copies/mL
-Sensitivity
5× 103 copies/mL
Caution:
1. This product is FOR RESEARCH USE ONLY!
Technical Principle & Methodology
The quality assurance and experimental performance of this biological reagent are rigorously optimized and standardized according to its specific technical pathway:
TaqMan Probe or SYBR Green Chimeric Dye Amplification
(TaqMan探针法或SYBR Green荧光染料嵌合扩增技术). This ensures maximum reproducibility across clinical research and scientific workflows.
