Anti-VEGF antibody

Product Information

Product Name: Anti-VEGF antibody

Catalog No.: LSMab09933

Packing: 100ug

Form: liquid

Purity:more than 95% as determined by SDS-PAGE

Purification: protein A+G purified

Host: Mouse

Isotype: IgG2b

Storage Condition: 0.01MPBS pH 7.4,-20degrees for 24 months(Avoid repeated freeze / thaw cycles.)

Immunogen Information

Immunogen Name:vascular endothelial growth factor A

Background: VEGFA, also named as VEGF or VPF, belongs to the PDGF/VEGF growth factor family. It is a growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. VEGFA induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. It binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Defects in VEGFA are a cause of susceptibility to microvascular complications of diabetes type 1(MVCD1). VEGFA has 13 isoforms with MW 16kd to 45kd. Some isoforms has homodimer form, such as VEGFA189(38kd), VEFGA110(34kd). VEGFA is a 34kd to 42kd, dimeric, disulfide-bound glycoprotein. VEGF-A exists in at least seven homodimeric isoforms. The monomers consist of 121, 145, 148, 165, 183, 189, or 206 amino acids(PMID:15602010). This antibody can recognize all the isoforms of VEFGA.

Synonyms: L VEGFA, MVCD1, Vascular permeability factor, VEGF, VEGF A, VEGFA, VPF

Calculated MW: 45 kDa

Uniprot ID: P15692

Gene ID:

Validation

Specificity: Human

Tested Application: ELISA, WB, IHC

Recommended dilution: WB: 1:1000-1:4000; IHC: 1:50-1:500

IHC use: Immunohistochemistry of paraffin-embedded human stomach cancer tissue slide using LSMab09933 (VEGF antibody) at dilution of 1:200

WB use: MCF-7 cells were subjected to SDS PAGE followed by western blot with LSMab09933 (VEGF antibody) at dilution of 1:2000

Caution:

1. Product is used for research only!