Anti-ATP5A1 antibody

Product Name Anti-ATP5A1 antibody

Catalog No PAab00703

Packing 100ug

Form liquid

Alternative Name ATP5A antibody, ATP5AL2 antibody, ATPM

Purification Protein A+G purification

Purity 95% as determined by SDS-PAGE

Host Mouse

Isotype IgG1

Storage PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20Centigrade for 24 months (Avoid repeated freeze / thaw cycles.)

Background/ FUNCTION

Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. Subunit alpha does not bear the catalytic high-affinity ATP-binding sites (By similarity).

Immunogen ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle

Specificity Human, Mouse ,Rat, Monkey

Tested Application ELISA,IHC,IF,WB,FC

Recommended dilution

IHC use

Immunohistochemistry of paraffin-embedded human heart usingATP5A1 antibody at dilution of 1:50

WB use

Western blot analysis of ATP5A1 in various tissues and cell lines using Fine Test antibody PAab00703

Protein Information

Gene ID498

Uniprot ID P25705

Calculated MW

location