Anti-beta actin antibody

Product Name Anti-beta actin antibody

Catalog No PAab00871

Packing 100ug

Form liquid

Alternative Name

Purification Protein A+G purification

Purity 95% as determined by SDS-PAGE

Host Mouse

Isotype IgG2a

Storage PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20Centigrade for 24 months (Avoid repeated freeze / thaw cycles.)

Background/ FUNCTION

Beta actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 41 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody was generated against N-terminal region of human beta actin protein and can cross-react with other actins.

Immunogen actin, beta

Specificity Human

Tested Application ELISA

Recommended dilution

IHC use

Immunohistochemical of paraffin-embedded human colon using Anti-beta actin antibody at dilution of 1:50.

WB use

Western blot of Hela cell with anti-Actin-Beta at 1:1000-1:3000.

Protein Information

Gene ID60

Uniprot ID P60709

Calculated MW 42 kDa

location