Anti-BrdU antibody

Product Name Anti-BrdU antibody

Catalog No PAab00952

Packing 100ug

Form liquid

Alternative Name 5-bromo-2-deoxyuridine antibody, BrDu antibody, BrdUrd antibody, Bromodeoxyuridine antibody, BUdR

Purification Protein A+G purification

Purity 95% as determined by SDS-PAGE

Host Mouse

Isotype IgG2a

Storage PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20Centigrade for 24 months (Avoid repeated freeze / thaw cycles.)

Background/ FUNCTION

Bromodeoxyuridine (5-bromo-2'-deoxyuridine, BrdU, BUdR, BrdUrd) is a synthetic nucleoside that is an analog of thymidine. It can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication. As such, BrdU is used for birth dating and monitoring cell proliferation. BrdU is a toxic and mutagenic substance. It triggers cell death, the formation of teratomas, alters DNA stability, lengthens the cell cycle, and has mitogenic, transcriptional and translational effects on cells that incorporate it. This antibody does not cross react with Thymidine.

Immunogen compound

Specificity Human, Mouse ,Rat

Tested Application ELISA,IF,IHC

Recommended dilution IHC:1:200-1:1000;IF:1:50-1:500

IHC use

Immunohistochemistry of paraffin-embedded mouse spleen tissue slide (with/without BrdU feeding) using BrDu Antibody at dilution of 1:400. Heat mediated antigen retrieved with Tris-EDTA bufferpH9).

WB use

Protein Information

Gene ID

Uniprot ID

Calculated MW

location