Product Name Anti-BrdU antibody
Catalog No PAab00952
Alternative Name 5-bromo-2-deoxyuridine antibody, BrDu antibody, BrdUrd antibody, Bromodeoxyuridine antibody, BUdR
Purification Protein A+G purification
Purity 95% as determined by SDS-PAGE
Storage PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20Centigrade for 24 months (Avoid repeated freeze / thaw cycles.)
Bromodeoxyuridine (5-bromo-2'-deoxyuridine, BrdU, BUdR, BrdUrd) is a synthetic nucleoside that is an analog of thymidine. It can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication. As such, BrdU is used for birth dating and monitoring cell proliferation. BrdU is a toxic and mutagenic substance. It triggers cell death, the formation of teratomas, alters DNA stability, lengthens the cell cycle, and has mitogenic, transcriptional and translational effects on cells that incorporate it. This antibody does not cross react with Thymidine.
Specificity Human, Mouse ,Rat
Tested Application ELISA,IF,IHC
Recommended dilution IHC:1:200-1:1000;IF:1:50-1:500
Immunohistochemistry of paraffin-embedded mouse spleen tissue slide (with/without BrdU feeding) using BrDu Antibody at dilution of 1:400. Heat mediated antigen retrieved with Tris-EDTA bufferpH9).