Anti-MAGEA3 antibody

Product Name Anti-MAGEA3 antibody

Catalog No PAab04937

Packing 100ug

Form liquid

Alternative Name MAGE3

Purification Protein A+G purification

Purity 95% as determined by SDS-PAGE

Host Mouse

Isotype IgG2a

Storage PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20Centigrade for 24 months (Avoid repeated freeze / thaw cycles.)

Background/ FUNCTION

Proposed to enhance ubiquitin ligase activity of RING- type zinc finger-containing E3 ubiquitin-protein ligases. May enhance ubiquitin ligase activity of TRIM28 and stimulate p53/TP53 ubiquitination by TRIM28. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex. May play a role in embryonal development and tumor transformation or aspects of tumor progression. In vitro promotes cell viability in melanoma cell lines. Antigen recognized on a melanoma by autologous cytolytic T-lymphocytes.

Immunogen melanoma antigen family A, 3

Specificity Human

Tested Application ELISA,WB,IHC

Recommended dilution

IHC use

Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using MAGEA3 Antibody at dilution of 1:200 heat mediated antigen retrieved with Tris-EDTA bufferpH9).

WB use

mouse brain tissue were subjected to SDS PAGE followed by western blot with PAab04937( MAGEA3 Antibody) at dilution of 1:2000

Protein Information

Gene ID4102

Uniprot ID P43357

Calculated MW

location