Anti-TNF alpha antibody
Product Name Anti-TNF alpha antibody
Catalog No PAab08815
Packing 100ug
Form liquid
Alternative Name Cachectin antibody, DIF antibody, TNF antibody, TNF a antibody, TNF alpha antibody, TNFA antibody, TNF-a antibody, TNFSF2 antibody, Tumor necrosis factor
Purification Protein A+G purification
Purity 95% as determined by SDS-PAGE
Host Mouse
Isotype IgG1
Storage PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20Centigrade for 24 months (Avoid repeated freeze / thaw cycles.)
Background/ FUNCTION
TNF, as also known as TNF-alpha, or cachectin, is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. It is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-alpha converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.
Immunogen tumor necrosis factor (TNF superfamily, member 2)
Specificity Human, Mouse
Tested Application ELISA,WB,IHC
Recommended dilution WB : 1:500-1:5000 IHC : 1:200-1:1000
IHC use
Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using TNF-a Antibody at dilution of 1:800. heat mediated antigen retrieved with Tris-EDTA bufferpH9).
WB use
LPS treated RAW 264.7 cells were subjected to SDS PAGE followed by western blot with PAab08815( TNF-a Antibody) at dilution of 1:1000
Protein Information
Gene ID7124
Uniprot ID P01375
Calculated MW 22kDa
location
English