Mouse 5- hydroxytryptamine receptor 5A, Htr5a ELISA KIT

Product Name:Mouse 5- hydroxytryptamine receptor 5A, Htr5a ELISA KIT
Packing:96T

Catalog No.:ELI-24681m

Gene Name:Mouse Htr5a

Detect Range:78.1-5000pg/ml

Sensitivity:46.875pg/ml

Target Protein Name:Mouse Htr5a

Alternative Name:Mouse 5-hydroxytryptamine receptor 5A, Htr5a
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Mouse 5- hydroxytryptamine receptor 5A, Htr5a ELISA KIT allows for the in vitro quantitative determination of Mouse Htr5a concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Mouse 5- hydroxytryptamine receptor 5A, Htr5a ELISA KIT has been pre-coated with an Mouse 5-hydroxytryptamine receptor 5A, Htr5a antibody specific to Mouse Htr5a .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Htr5a and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Htr5a , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Htr5a in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!