Mouse A- kinase anchor protein 12, Akap12 ELISA KIT

Product Name:Mouse A- kinase anchor protein 12, Akap12 ELISA KIT
Packing:96T

Catalog No.:ELI-35127m

Gene Name:Mouse Akap12

Detect Range:0.156-10ng/ml

Sensitivity:0.094ng/ml

Target Protein Name:Mouse Akap12

Alternative Name:Mouse A-kinase anchor protein 12, Akap12
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Mouse A- kinase anchor protein 12, Akap12 ELISA KIT allows for the in vitro quantitative determination of Mouse Akap12 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Mouse A- kinase anchor protein 12, Akap12 ELISA KIT has been pre-coated with an Mouse A-kinase anchor protein 12, Akap12 antibody specific to Mouse Akap12 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Akap12 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Akap12 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Akap12 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!