Mouse A- kinase anchor protein 8, Akap8 ELISA KIT

Product Name:Mouse A- kinase anchor protein 8, Akap8 ELISA KIT
Packing:96T

Catalog No.:ELI-11941m

Gene Name:Mouse Akap8

Detect Range:78-5000pg/ml

Sensitivity:46.9pg/ml

Target Protein Name:Mouse Akap8

Alternative Name:Mouse A-kinase anchor protein 8, Akap8
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Mouse A- kinase anchor protein 8, Akap8 ELISA KIT allows for the in vitro quantitative determination of Mouse Akap8 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Mouse A- kinase anchor protein 8, Akap8 ELISA KIT has been pre-coated with an Mouse A-kinase anchor protein 8, Akap8 antibody specific to Mouse Akap8 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Akap8 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Akap8 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Akap8 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!