Mouse Ankyrin repeat and SOCS box protein 14, Asb14 ELISA KIT

Product Name:Mouse Ankyrin repeat and SOCS box protein 14, Asb14 ELISA KIT
Packing:96T

Catalog No.:ELI-11269m

Gene Name:Mouse Asb14

Detect Range:15.62-1000pg/ml

Sensitivity:9.375pg/ml

Target Protein Name:Mouse Asb14

Alternative Name:Mouse Ankyrin repeat and SOCS box protein 14, Asb14
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Mouse Ankyrin repeat and SOCS box protein 14, Asb14 ELISA KIT allows for the in vitro quantitative determination of Mouse Asb14 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Mouse Ankyrin repeat and SOCS box protein 14, Asb14 ELISA KIT has been pre-coated with an Mouse Ankyrin repeat and SOCS box protein 14, Asb14 antibody specific to Mouse Asb14 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Asb14 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Asb14 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Asb14 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!