Mouse AP- 3 complex subunit beta- 2, Ap3b2 ELISA KIT
Product Name:Mouse AP- 3 complex subunit beta- 2, Ap3b2 ELISA KIT
Packing:96T
Catalog No.:ELI-34847m
Gene Name:Mouse Ap3b2
Detect Range:15.62-1000pg/ml
Sensitivity:9.375pg/ml
Target Protein Name:Mouse Ap3b2
Alternative Name:Mouse AP-3 complex subunit beta-2, Ap3b2
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse AP- 3 complex subunit beta- 2, Ap3b2 ELISA KIT allows for the in vitro quantitative determination of Mouse Ap3b2 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse AP- 3 complex subunit beta- 2, Ap3b2 ELISA KIT has been pre-coated with an Mouse AP-3 complex subunit beta-2, Ap3b2 antibody specific to Mouse Ap3b2 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Ap3b2 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Ap3b2 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Ap3b2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!