Mouse Apelin- 36 , APLN ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!
Synonyms
Apelin-36,;
Search name
Mouse Apelin-36 ELISA Kit,Apelin-36 ELISA Kit, APLN36 ELISA Kit, Mouse APLN ELISA Kit
Intended use
This immunoassay kit allows for the in vitro quantitative determination of mouse Apelin-36 concentrations in serum, Plasma, tissue
homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Apelin-36. Standards or samples are then added
to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Apelin-36 and Avidin
conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to
each well. Only those wells that contain Apelin-36, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color.
The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Apelin-36 in the samples is then determined by comparing
the O.D. of the samples to the standard curve.
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