Mouse Bckdhb ELISA KIT

Product Name:Mouse Bckdhb ELISA KIT
Packing:96T

Catalog No.:ELI-13291m

Gene Name:Mouse Bckdhb

Detect Range:31.2-2000pg/ml

Sensitivity:18.75pg/ml

Target Protein Name:Mouse Bckdhb

Alternative Name:Mouse Bckdhb
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Mouse Bckdhb ELISA KIT allows for the in vitro quantitative determination of Mouse Bckdhb concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Mouse Bckdhb ELISA KIT has been pre-coated with an Mouse Bckdhb antibody specific to Mouse Bckdhb .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Bckdhb and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Bckdhb , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Bckdhb in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!