Mouse BRCA1- associated protein, Brap ELISA KIT

Product Name:Mouse BRCA1- associated protein, Brap ELISA KIT
Packing:96T

Catalog No.:ELI-50133m

Gene Name:Mouse Brap

Detect Range:0.15-10ng/ml

Sensitivity:0.094ng/ml

Target Protein Name:Mouse Brap

Alternative Name:Mouse BRCA1-associated protein, Brap
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Mouse BRCA1- associated protein, Brap ELISA KIT allows for the in vitro quantitative determination of Mouse Brap concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Mouse BRCA1- associated protein, Brap ELISA KIT has been pre-coated with an Mouse BRCA1-associated protein, Brap antibody specific to Mouse Brap .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Brap and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Brap , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Brap in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!