Mouse C- telopeptide of type II collagen ELISA Kit

Product Name:Mouse C- telopeptide of type II collagen ELISA Kit
Packing:96T

Catalog No.:ELA-E0686m

Gene Name:Mouse CTX-II

Detect Range:15.6-1000pg/ml

Sensitivity:9.375pg/ml

Target Protein Name:Mouse CTX-II

Alternative Name:Mouse C-telopeptide of type II collagen
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Mouse C- telopeptide of type II collagen ELISA Kit allows for the in vitro quantitative determination of Mouse CTX-II concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Mouse C- telopeptide of type II collagen ELISA Kit has been pre-coated with an Mouse C-telopeptide of type II collagen antibody specific to Mouse CTX-II .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse CTX-II and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse CTX-II , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse CTX-II in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!