Mouse Calcitonin, CT ELISA Kit
96 Tests
Operating instructions

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
Calcitonin,thyrocalcitonin,CALCA; CALC1; CGRP; CGRP-I; CGRP1; CT; KC, Calcitonin,alpha-type CGRP; CGRP-I; CGRP; CALC1;
CGRP1; Calcitonin 1; calcitonin gene-related peptide I; calcitonin/calcitonin-related polypeptide, alpha; katacalcin; calcitonin-related
polypeptide alpha

Search name
Mouse Calcitonin ELISA Kit, Mouse calcitonin gene related peptide ELISA Kit, Mouse CGRP ELISA Kit, Mouse thyrocalcitonin
ELISA Kit, Mouse CALCA ELISA Kit, Mouse CALC1 ELISA Kit, Mouse CGRP-I ELISA Kit, Mouse CGRP1 ELISA Kit, Mouse CT
ELISA Kit, Mouse KC ELISA Kit, Mouse Calcitonin,alpha-type CGRP ELISA Kit, Mouse Calcitonin 1 ELISA Kit, Mouse calcitonin
gene-related peptide I ELISA Kit, Mouse calcitonin/calcitonin-related polypeptide alpha ELISA Kit, Mouse katacalcin ELISA Kit

Intended use
This immunoassay kit allows for the in vitro quantitative determination of mouse Calcitonin concentrations in serum, plasma, tissue
homogenates, cell culture supernates, and other biological fluids.

Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been
pre-coated with an antibody specific to Calcitonin, During the reaction, Calcitonin in the sample or standard competes with a fixed amount
of biotin-labeled Calcitonin for sites on a pre-coated Monoclonal antibody specific to Calcitonin. Excess conjugate and unbound sample or
standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and
incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a
sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of
Calcitonin in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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