Mouse Carboxymethylenebutenolidase homolog, Cmbl ELISA KIT

Product Name:Mouse Carboxymethylenebutenolidase homolog, Cmbl ELISA KIT
Packing:96T

Catalog No.:ELI-10200m

Gene Name:Mouse Cmbl

Detect Range:0.15-10ng/ml

Sensitivity:0.094ng/ml

Target Protein Name:Mouse Cmbl

Alternative Name:Mouse Carboxymethylenebutenolidase homolog, Cmbl
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Mouse Carboxymethylenebutenolidase homolog, Cmbl ELISA KIT allows for the in vitro quantitative determination of Mouse Cmbl concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Mouse Carboxymethylenebutenolidase homolog, Cmbl ELISA KIT has been pre-coated with an Mouse Carboxymethylenebutenolidase homolog, Cmbl antibody specific to Mouse Cmbl .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Cmbl and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Cmbl , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Cmbl in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!