Mouse Carboxypeptidase B2, CPB2 ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Carboxypeptidase B2,Thrombin-activable fibrinolysis inhibitor,TAFI,Carboxypeptidase U,CPU,Plasma carboxypeptidase B,pCPB, CPB2,TAFI; CPU; PCPB; carboxypeptidase B-like protein; carboxypeptidase B2; carboxypeptidase B2,plasma carboxypeptidase U;carboxypeptidase R; thrombin-activable fibrinolysis inhibitor; thrombin-activatable fibrinolysis inhibitor; carboxypeptidase B2
Search name
Mouse Carboxypeptidase B2 ELISA KIT ,Mouse Thrombin-activable fibrinolysis inhibitor ELISA KIT ,Mouse TAFI ELISA KIT ,Mouse Carboxypeptidase U ELISA KIT ,Mouse CPU ELISA KIT ,Mouse Plasma carboxypeptidase B ELISA KIT ,Mouse pCPB ELISA KIT ,Mouse CPB2 ELISA KIT ,Mouse TAFI ELISA KIT ,Mouse CPU ELISA KIT ,Mouse PCPB ELISA KIT ,Mouse carboxypeptidase B-like protein ELISA KIT ,Mouse carboxypeptidase B2 ELISA KIT ,Mouse carboxypeptidase B2 ELISA KIT ,Mouse plasma carboxypeptidase U;carboxypeptidase R ELISA KIT ,Mouse thrombin-activable fibrinolysis inhibitor ELISA KIT ,Mouse thrombin-activatable fibrinolysis inhibitor ELISA KIT ,Mouse carboxypeptidase B2 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse Carboxypeptidase B2 concentrations in serum, Plasma, Urine, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Carboxypeptidase B2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Carboxypeptidase B2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Carboxypeptidase B2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Carboxypeptidase B2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.