Mouse CDC42 small effector protein 1, CDC42SE1 ELISA Kit
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
CDC42SE1,SCIP1; SPEC1; 1300002M12Rik; CDC42 small effector protein 1; CDC42-binding protein SCIP1; signaling molecule SPEC1 beta; small effector of CDC42 protein 1; small protein effector 1 of Cdc42; CDC42 small effector 1
Search name
Mouse CDC42SE1 ELISA KIT ,Mouse SCIP1 ELISA KIT ,Mouse SPEC1 ELISA KIT ,Mouse 1300002M12Rik ELISA KIT ,Mouse CDC42 small effector protein 1 ELISA KIT ,Mouse CDC42-binding protein SCIP1 ELISA KIT ,Mouse signaling molecule SPEC1 beta ELISA KIT ,Mouse small effector of CDC42 protein 1 ELISA KIT ,Mouse small protein effector 1 of Cdc42 ELISA KIT ,Mouse CDC42 small effector 1 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse CDC42 small effector protein 1, CDC42SE1 concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique.The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CDC42 small effector protein 1, CDC42SE1, During the reaction, Mouse CDC42 small effector protein 1, CDC42SE1 in the sample or standard competes with a fixed amount of biotin-labeled Mouse CDC42 small effector protein 1, CDC42SE1 for sites on a pre-coated Monoclonal antibody specific to Mouse CDC42 small effector protein 1, CDC42SE1. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Mouse CDC42 small effector protein 1, CDC42SE1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.