Mouse Centrosomal protein of 135 kDa, Cep135 ELISA KIT

Product Name:Mouse Centrosomal protein of 135 kDa, Cep135 ELISA KIT
Packing:96T

Catalog No.:ELI-50360m

Gene Name:Mouse Cep135

Detect Range:31.2-2000pg/ml

Sensitivity:18.75pg/ml

Target Protein Name:Mouse Cep135

Alternative Name:Mouse Centrosomal protein of 135 kDa, Cep135
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Mouse Centrosomal protein of 135 kDa, Cep135 ELISA KIT allows for the in vitro quantitative determination of Mouse Cep135 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Mouse Centrosomal protein of 135 kDa, Cep135 ELISA KIT has been pre-coated with an Mouse Centrosomal protein of 135 kDa, Cep135 antibody specific to Mouse Cep135 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Cep135 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Cep135 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Cep135 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!