Mouse Coagulation factor VII, F7 ELISA KIT

Product Name:Mouse Coagulation factor VII, F7 ELISA KIT
Packing:96T

Catalog No.:ELI-06015m

Gene Name:Mouse F7

Detect Range:15.6-1000pg/ml

Sensitivity:9.375pg/ml

Target Protein Name:Mouse F7

Alternative Name:Mouse Coagulation factor VII, F7
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Mouse Coagulation factor VII, F7 ELISA KIT allows for the in vitro quantitative determination of Mouse F7 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Mouse Coagulation factor VII, F7 ELISA KIT has been pre-coated with an Mouse Coagulation factor VII, F7 antibody specific to Mouse F7 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse F7 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse F7 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse F7 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!