Mouse Coagulation factor VIII, F8 ELISA KIT

Product Name:Mouse Coagulation factor VIII, F8 ELISA KIT
Packing:96T

Catalog No.:ELI-02864m

Gene Name:Mouse F8

Detect Range:78.1-5000pg/ml

Sensitivity:46.9pg/ml

Target Protein Name:Mouse F8

Alternative Name:Mouse Coagulation factor VIII, F8
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Mouse Coagulation factor VIII, F8 ELISA KIT allows for the in vitro quantitative determination of Mouse F8 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Mouse Coagulation factor VIII, F8 ELISA KIT has been pre-coated with an Mouse Coagulation factor VIII, F8 antibody specific to Mouse F8 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse F8 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse F8 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse F8 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!