Mouse Cofilin 1,Non- Muscle ELISA Kit
Product Name:Mouse Cofilin 1,Non- Muscle ELISA Kit
Packing:96T
Catalog No.:ELA-E1525m
Gene Name:Mouse CFL1
Detect Range:15.62-1000pg/ml
Sensitivity:9.375pg/ml
Target Protein Name:Mouse CFL1
Alternative Name:Mouse Cofilin 1,Non-Muscle
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Cofilin 1,Non- Muscle ELISA Kit allows for the in vitro quantitative determination of Mouse CFL1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Cofilin 1,Non- Muscle ELISA Kit has been pre-coated with an Mouse Cofilin 1,Non-Muscle antibody specific to Mouse CFL1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse CFL1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse CFL1 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse CFL1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!