Mouse Cytosolic 5’- nucleotidase 1A, NT5C1A ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

NT5C1A,CN-I; CN-IA; CN1; CN1A; CNI; AMP-specific 5-NT; cytosolic 5 nucleotidase, type 1A; cytosolic 5-nucleotidase 1A; cytosolic 5-nucleotidase IA; 5-nucleotidase, cytosolic IA

Search name

Mouse NT5C1A ELISA KIT ,Mouse CN-I ELISA KIT ,Mouse CN-IA ELISA KIT ,Mouse CN1 ELISA KIT ,Mouse CN1A ELISA KIT ,Mouse CNI ELISA KIT ,Mouse AMP-specific 5-NT ELISA KIT ,Mouse cytosolic 5 nucleotidase ELISA KIT ,Mouse  type 1A ELISA KIT ,Mouse cytosolic 5-nucleotidase 1A ELISA KIT ,Mouse cytosolic 5-nucleotidase IA ELISA KIT ,Mouse 5-nucleotidase cytosolic IA ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Mouse Cytosolic 5’- nucleotidase 1A, NT5C1A concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to NT5C1A. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for NT5C1A and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain NT5C1A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of NT5C1A in the samples is then determined by comparing the O.D. of the samples to the standard curve.