Mouse Cytosolic 5'- nucleotidase 1B, Nt5c1b ELISA KIT

Product Name:Mouse Cytosolic 5'- nucleotidase 1B, Nt5c1b ELISA KIT
Packing:96T

Catalog No.:ELI-12307m

Gene Name:Mouse Nt5c1b

Detect Range:78.1-5000pg/ml

Sensitivity:46.875pg/ml

Target Protein Name:Mouse Nt5c1b

Alternative Name:Mouse Cytosolic 5'-nucleotidase 1B, Nt5c1b
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Mouse Cytosolic 5'- nucleotidase 1B, Nt5c1b ELISA KIT allows for the in vitro quantitative determination of Mouse Nt5c1b concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Mouse Cytosolic 5'- nucleotidase 1B, Nt5c1b ELISA KIT has been pre-coated with an Mouse Cytosolic 5'-nucleotidase 1B, Nt5c1b antibody specific to Mouse Nt5c1b .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Nt5c1b and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Nt5c1b , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Nt5c1b in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!