Mouse Defensin Alpha 5, Paneth Cell Specific ELISA Kit

Product Name:Mouse Defensin Alpha 5, Paneth Cell Specific ELISA Kit
Packing:96T

Catalog No.:ELA-E1718m

Gene Name:Mouse DEFa5

Detect Range:15.6-1000pg/ml

Sensitivity:9.375pg/ml

Target Protein Name:Mouse DEFa5

Alternative Name:Mouse Defensin Alpha 5, Paneth Cell Specific
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Mouse Defensin Alpha 5, Paneth Cell Specific ELISA Kit allows for the in vitro quantitative determination of Mouse DEFa5 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Mouse Defensin Alpha 5, Paneth Cell Specific ELISA Kit has been pre-coated with an Mouse Defensin Alpha 5, Paneth Cell Specific antibody specific to Mouse DEFa5 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse DEFa5 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse DEFa5 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse DEFa5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!