Mouse Dentin sialophosphoprotein, DSPP ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Dentin sialophosphoprotein, Dentin sialoprotein,DSP, DSPP,DSP; DFNA39; DGI1; DMP3; DPP; DTDP2; dentin phosphophoryn; dentin phosphoprotein; dentin phosphoryn;

Search name

Mouse Dentin sialophosphoprotein ELISA KIT ,Mouse Dentin sialoprotein ELISA KIT ,Mouse DSP ELISA KIT ,Mouse  DSPP ELISA KIT ,Mouse DSP ELISA KIT ,Mouse DFNA39 ELISA KIT ,Mouse DGI1 ELISA KIT ,Mouse DMP3 ELISA KIT ,Mouse DPP ELISA KIT ,Mouse DTDP2 ELISA KIT ,Mouse dentin phosphophoryn ELISA KIT ,Mouse dentin phosphoprotein ELISA KIT ,Mouse dentin phosphoryn ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Mouse Dentin sialophosphoprotein concentrations in serum, Plasma, Urine, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dentin sialophosphoprotein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Dentin sialophosphoprotein and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Dentin sialophosphoprotein, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Dentin sialophosphoprotein in the samples is then determined by comparing the O.D. of the samples to the standard curve.