Mouse Deoxycytidylate deaminase, Dctd ELISA KIT
Product Name:Mouse Deoxycytidylate deaminase, Dctd ELISA KIT
Packing:96T
Catalog No.:ELI-08983m
Gene Name:Mouse Dctd
Detect Range:15.6-1000pg/ml
Sensitivity:9.375pg/ml
Target Protein Name:Mouse Dctd
Alternative Name:Mouse Deoxycytidylate deaminase, Dctd
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Deoxycytidylate deaminase, Dctd ELISA KIT allows for the in vitro quantitative determination of Mouse Dctd concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Deoxycytidylate deaminase, Dctd ELISA KIT has been pre-coated with an Mouse Deoxycytidylate deaminase, Dctd antibody specific to Mouse Dctd .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Dctd and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Dctd , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Dctd in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!