Mouse DNA fragmentation factor subunit beta, DFFB ELISA Kit

96 Tests

Operating instructions

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Dffb,CAD; CPAN; DFF-40; DFF2; DFF40; DNA fragmentation factor 40 kDa subunit; DNA fragmentation factor subunit beta; caspase-activated DNase; caspase-activated deoxyribonuclease; caspase-activated nuclease; DNA fragmentation factor 40kDa beta polypeptide,caspase-activated DNase

Search name

Mouse Dffb ELISA KIT ,Mouse CAD ELISA KIT ,Mouse CPAN ELISA KIT ,Mouse DFF-40 ELISA KIT ,Mouse DFF2 ELISA KIT ,Mouse DFF40 ELISA KIT ,Mouse DNA fragmentation factor 40 kDa subunit ELISA KIT ,Mouse DNA fragmentation factor subunit beta ELISA KIT ,Mouse caspase-activated DNase ELISA KIT ,Mouse caspase-activated deoxyribonuclease ELISA KIT ,Mouse caspase-activated nuclease ELISA KIT ,Mouse DNA fragmentation factor 40kDa beta polypeptide ELISA KIT ,Mouse caspase-activated DNase ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Mouse DNA fragmentation factor subunit beta, DFFB concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

Test principle

The ELISA is based on the competitive binding enzyme immunoassay technique.The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse DNA fragmentation factor subunit beta, DFFB, During the reaction, Mouse DNA fragmentation factor subunit beta, DFFB in the sample or standard competes with a fixed amount of biotin-labeled Mouse DNA fragmentation factor subunit beta, DFFB for sites on a pre-coated Monoclonal antibody specific to Mouse DNA fragmentation factor subunit beta, DFFB. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Mouse DNA fragmentation factor subunit beta, DFFB in the samples is then determined by comparing the O.D. of the samples to the standard curve.